ABSTRACT
Introduction: In December 2019, a rapid spread of highly infectious, Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2), was reported in Wuhan, China. The gold standard for diagnosis of SARS-CoV-2 infection is nucleic acid amplification technology by detecting its viral Ribonucleic Acid (RNA) from respiratory swabs (oropharyngeal and nasopharyngeal) by Reverse Transcription-Polymerase Chain Reaction (RT-PCR) whose specificity is approximately 95%. Magnetic bead RNA extraction was benchmarked against the commercial QIAcube extraction platform. Aim(s): To compare the efficacy of nucleic acid extraction by manual method and automated magnetic bead-based method to detect SARS-CoV-2. Material(s) and Method(s): The present observational comparative study was conducted in the Department of Microbiology, Autonomous State Medical College, Firozabad, Uttar Pradesh, India. Duration of the study was from February to March 2022. A total of 470 oropharyngeal and nasopharyngeal samples were included in this study to observe the efficacy of nucleic acid extraction by manual extraction and automated extraction for SARS-CoV-2. Data were entered in Microsoft Excel software and analysed using Statistical Package for the Social Sciences (SPSS) version 26.0. Result(s): During this study period, a total of 470 individual samples were tested in 94 pools. Out of these 470 individuals, 331 were males (70.5%) and 139 were females (29.5%). All 94 pools were found negative by both automatic and manual extraction methods. Envelope (E) gene was found in one pool (1.06%) by the manual RNA extraction method. E gene was absent in 93 pools (98.94%) by manual method. Internal control was found highest in 88 pools (93.62%) by the automated extraction method. Conclusion(s): Automated workflows avoid human error from the sample processing pipeline and also ensure as well as enhance the meaningful output, diagnostic precision, and testing capacity. Automated instruments are in wide usage because of their capability of processing thousands of samples per day with the support of minimal staff. Copyright © 2022 Journal of Clinical and Diagnostic Research. All rights reserved.
ABSTRACT
Covid-19 pandemic has affected every aspect of life worldwide, includes health sector, service sector, hospitality sector, tour & travel sector, and more specially the education sector at school level as well as at college level of every country. Affect on education sector indirectly affected the upcoming future of nation. Due to infectious nature of disease, people are not supposed to expose outside and come in touch and also instructed to keep distance and follow covid protocol in order to prevent the spread of virus. In such situation we have to lay additional effort to save our kids and decide not to send them to school/college. Management of the school/college is also not in state, taking any risk with student’s life and looking toward another alternative of teaching. We are forced to rethink how education works will carry on in the wake of COVID-19 and consider pandemic as an opportunity to reimaging learning and equip students with skills required navigating the future. This is where new teaching pedagogy and technology comes in play and impacts the whole scenario of education and also open new door of business as an opportunity to reimaging learning and modernize our education systems for the 21st century. This paper presents the analysis of impact of pandemic on traditional smooth running education system and new digital learning platform as the solution of mythic “education is broken” in pandemic. Also present challenges and problems occur due to rapid adoption of online digital learning system whose design is not fully tested to be best pedagogical practices. Paper presents future prospects of remote learning by expert and also brings the new business opportunity in education sector.